How should I acquire my NMR spectra?
8 February 2016 Comments Off on How should I acquire my NMR spectra? FAQ - Sample Preparation
You will get the best results analyzing spectra acquired with NMR parameters similar to those used to build the metabolite library. The Chenomx metabolite library was acquired between pH 4 and 9 at a temperature of 298 K (25 °C), with an acquisition time of 4 s and recycling delay of 1 s. The recycle delay includes a 990 ms saturation pulse on water. Our 1D pulse sequence is NOE-based, with a short mixing time of 100 ms. The mixing time also includes a water saturation pulse. The length of the proton 90° pulse (pw) was calibrated to maximize the intensity of the DSS peak.